What is a blotting?

As an analytical technique for biomolecules such as DNA, RNA and protein etc., the blotting technology is a technology of detecting using a probe that specifically binds to the target molecule.

What is an immune blot?

A blotting technology for detecting proteins in a sample using an antibody as a probe. Sample proteins are separated by molecular weight using electrophoresis and transferred to the membrane. An antibody specific for the target protein in the sample is bound to perform the identification and quantitative analysis.

• Because of the high reliability of the analysis results despite the high proportion of manual work, it is used in general laboratory experiments.
• It is also used to diagnose diseases due to the antibody specificity and analytical reliability of the immunoblot

Problems of traditional immunoblot

The sample processing efficiency is low due to manual work, and it takes a long time to analyze due to the antibody reaction time.

Trends of immunoblot (integration / automation / cartridge-based assays)

Overview of T-Mac technology development

• Reduction of antibody reaction time
- To shorten the antibody reaction time, which accounts for 85% of the time required for immunoblotting, the principle of CDR (Cyclic Draining and Replenishing) was first applied to the immunoblot.

• Multi Analysis
- Minimization of protein separation distance and time through optimized processing know-how of sample injection groove
- Implementation of multi-sample analysis (> 384) of existing high-capacity analysis equipment for the first time in immunoblot

• Minimizing reagent use
- It is possible to reduce the reagent and cleaning solution usage by 70% (cost reduction) compared to the conventional method by minimizing the reaction space of the reagent containing expensive antibody and the use space of the washing solution.

• Summary of technical advantages
Characterized by high density and rapid immunoblot, the Micro Rapid Immunblot Array System of T-Mac Co., Ltd. enables high-efficiency antibody studies.
Therefore, it is possible to conduct simultaneous study of multiple antibodies and multiple samples as well as basic research fields of research by universities and research institutes, having the advantages in the fields of diagnosis and new drug research.